Biological and Serological Properties of  Tomato Yellow Leaf Curl Virus (TYLCV)

A. Al-Fadhal, Fadhal

	Biological and Serological Properties of Tomato Yellow Leaf Curl Virus (TYLCV)
Magazine of Al-Kufa University for Biology
Article 1, Volume 4, Issue 2, 2012, Pages 139-145
Author
Fadhal A. Al-Fadhal
Abstract
This work was conducted to determine the serological and biological characteristics of Tomato yellow leaf curl virus in plastic houses. Samples from tomato plants showing symptoms of leaf curling and rolling, plant stunting and deformation accompanied with leaf edge and interveinal chlorosis suspected to be of Tomato yellow leaf curl virus were collected. Test plants were inoculated with the virus by whiteflies Bemisia tabaci. Results showed that the host range of the virus was restricted to some host plants. The virus caused leaf rolling, vein clearing, and new leaves deformation of Datura stramonium and Solanum nigrum after 20 days of inoculation. Chenopodium amaranticolor, Phaseolus vulgaris, and Nicotiana glutinosa were responded to virus infection without obvious symptoms on the infected plants. The virus was detected in these plants by serological-ELISA test. These hosts may be representing source of virus transmitted to tomato plants by Bemisia tabaci. It has been found that the virus transmitted efficiently from infected to healthy plants by Bemisia tabaci. One insect found to transmit the virus by 25%, attained to 100% with 10 insect/plant. The minimum access period on infected plant to acquire the virus was found to be 30 min, and similar period for viruliferous Bemisia tabaci on healthy plant for inoculation. The incubation period of the virus in the insect was found to be 24 hrs. The increasing of access period of insect on both infected and healthy plants caused increasing in the efficacy of virus transmission.

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