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SPECTROPHOTOMETRIC DETERMINATION OF AMOXICILLIN IN PHARMACEUTICAL PREPARATIONS THROUGH DIAZOTIZATION AND COUPLING REACTION | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 53, Issue 4, 2012, Pages 713-723 | ||
| Author | ||
| Wasan A. Al-Uzri | ||
| Abstract | ||
| Two simple, rapid and sensitive spectrophotometric methods have been developed for the determination of amoxicillin (AMX) in pure form and pharmaceutical preparations. The proposed methods are based on a coupling reaction between AMX and diazotized p-amino benzoic acid or diazotized procain in alkaline medium to form an intense yellow, water-soluble dyes that are stable and have a maximum absorption at 435 nm using diazotized p- amino benzoic acid and 450 nm on using diazotized procain. The calibration graphs were linear over the concentration rangs of 0.4 to 10 µg mL-1 and 0.4 to 14 µg mL-1 with a limit of detection (LOD) of 0.1877 µg mL-1 and 0.1916 µg mL-1,molar absorbtivity of 1.914×104 L mol-1cm-1 and 2.544×104 L mol-1cm-1 , Sandellʼs sensitivity of 21.912×10-3 µg ml-1 and 16.486 ×10-3 µg ml-1 for diazotized p-amino benzoic acid and diazotized procain, respectively. The proposed methods were successfully applied to the determination of AMX in capsules and injections. | ||
| Keywords | ||
| spectrophotometry; diazotized p; amino benzoic acid; diazotized procain; Amoxicillin; pharmaceutical analysis | ||
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