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Purification of Crystals Protein by Aqueous Two Phase System from Bacillus thuringiensis Local Isolate | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 53, Issue 4, 2012, Pages 799-806 | ||
| Authors | ||
| Mohammad Adil Jaafar; Abdul Karrm Al-Kazaz; Ali Sadiq Mohammad | ||
| Abstract | ||
| The crystals protein of local isolate Bacillus thuringiensis were purified by using aqueous two phase system method in two steps ; the first 94% of crystals protein and spores were separated from cell dibbers by using 14% g/ml ammonium sulphate and 4% g/ml of Polyethylene glycol 6000 at pH=7 with separation time of 30 min. While in the second step, crystals protein were purified from spores by using a system consisted of 8% g/ml Dextran 60000-90000 and 9% g/ml of Polyethylene glycol 6000 at pH=7 with separation time of 2.5 h. the a final yield was 70.6% with purity of 92% . | ||
| Keywords | ||
| Aqueous; two phase system; protein purification | ||
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