SEROLOGICAL AND BIOLOGICAL IDENTIFICATION OF FOUR CUCUMBER MOSAIC VIRUS STRAINS AND ITS RELATION FOR BATHOGENESIS

	SEROLOGICAL AND BIOLOGICAL IDENTIFICATION OF FOUR CUCUMBER MOSAIC VIRUS STRAINS AND ITS RELATION FOR BATHOGENESIS
The Iraqi Journal of Agricultural Science
Article 1, Volume 39, Issue 1, 2008, Pages 59-68
Abstract
This study was undertaken to determine the existence of cucumber mosaic virus strain. Symptoms on indicator plants and serological characteristics were used to justify this goal. Symptoms manifested on cucumber , Cowpea (Vigna unguiculata Savi. Var Black eye) , globe amaranth (Gomphrena globosa L.) , Jimson weed (Datura stramonium L.).tobacco (Nicotiana tabacum Var Samsun). and Tomato (Lycopersicon esculentum Mill) indicated the presence of 4 different pathotypes , (1) weak , (2) mild , (3) severe , and (4) very severe .Partial purification of CMV from N. tabacum Var Samsun was achieved by grinding infected leaves in phosphate buffer . 0.5 M , pH 8 , containing 2% ascorbic acid and 1% Na-DIECA , and precipitating the virus by addition of 50% (W/V) ammonium sulfate. The absorption ratio of purified prepartions for the isolates (4,3,2.1) were found to be 1.73 , 1.5 , 1.52 and 1./ yielding 292 , 320 , 195 , and 246 mg/kg of virus particles respectively .Antisera for isolates with a titer of 1/1024 , and 1/2048 respectively were obtained to 1 and 3 strains by 4 intramuscular injections of purified virus preparation at 2.4 and 3.2 mg ml . The first 3 injections were weekly while the 4n injection was performed 2 weeks later.Double immunodiffusion test in agarose gel for the virus showed clear precipitation lines between wells containing the AS and those containing purified virus or leaf extracts from plants inoculated by the 4 isolates . According to these precipitation lines and spurs 4 strains of the virus were identified .

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