Spectrophotometric and High Performance Liquid Chromatographic Methods for Determination of Metoclopramide inPharmaceutical Preparations

A. Khaleel, Nada; Sh. Mahmood, Hana; S. Othman, Nabeel

doi:10.33899/rjs.2011.6510

	Spectrophotometric and High Performance Liquid Chromatographic Methods for Determination of Metoclopramide inPharmaceutical Preparations
Rafidain Journal of Science
Article 4, Volume 22, Issue 5, 2011, Pages 39-56 PDF (0 K)
DOI: 10.33899/rjs.2011.6510
Authors
Nada A. Khaleel; Hana Sh. Mahmood; Nabeel S. Othman
Abstract
A rapid, simple, and sensitive spectrophotometric and high performance liquid chromatographic methods have been developed for the quantitative determination of metoclopramide hydrochloride MCP in both pure and dosage forms. The spectrophotometric method is based on diazotization of MCP and then coupling with 2,4-dihydroxyacetophenone in alkaline medium. The resulting azodye exhibits maximum absorption at 450 nm with a molar absorptivity of 2.48 X 104 l.mol-1.cm-1. Beer's law is obeyed over the range 10-300µg/25 ml, i.e., 0.4-12 ppm with a relative standard deviation (RSD)of better than±1.092% and relative error better than -1.1%. HPLC method has been developed for the measurement of MCP, the analysis was achieved on a C8 column using acetonitrile, water, and methanol in the ratio of 40:50:10 (V:V:V) (in the presence of 1% of sodium acetate) as a mobile phase by isocratic elution at 1 ml/min. flow rate, and detection was done spectrophotometrically at 308 nm. A linear relationship is obeyed over the range 0.18-0.8 ppm with a relative standard deviation (RSD) of better than 3.7% and relative error better than -1.6%. Both methods were applied successfully to the assay of MCP in pharmaceutical preparations in the form of syrup, injection, and tablet.
Keywords
Metoclopramide; spectrophotometric methods; diazometry; HPLC; dosage form

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