A Study of Production Condition of the Polysaccharide from a Local Isolates  of the fungus Cladosporium herbarum  and Its Partial Identification

Ahmed Abid Al Juboory And  Abid Al Kareem Sulaiman Hassan Al Noaimy, Hawazin

	A Study of Production Condition of the Polysaccharide from a Local Isolates of the fungus Cladosporium herbarum and Its Partial Identification
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 12, Issue 4, 2012, Pages 1-7
Author
Hawazin Ahmed Abid Al Juboory and Abid Al kareem Sulaiman Hassan Al noaimy
Abstract
Abstract In this study , the production of polysaccharide from one of the local Cladosporium herbarum had been studied . and obtained from Mousel University / College of Education . At the beginning , we studied the preparation & selection of nutrient semi-synthetic media for the growth of the fungus , and facilitation of its production of polysaccharide The best production was obtained after 7 days of incubation in shaker incubator via (A) prepared medium which represents (P.D) medium . Then we studied the ability of the fungus to grow in liquid medium (P.D) in light and dark conditions . The growth of the fungus and the production of polysaccharides had been decreased in light compared with dark incubation conditions . Its effect had been studied in two types of media , stable media and media in shaking condition .Results showed that light decreased the growth of the fungus C. herbarum and The polysaccharides production in stable medium conditions , as the maximum production of polysaccharides in light and dark conditions were (0.67 gm / l and 1 gm / l ) respectively after 8 days of incubation while the Maximum production of polysaccharides in light and dark conditions in shaker incubation circumstances were (1.01 gm / l and 2.74 gm / l ) respectively .. On increasing the size of the medium from 50 ml for each 250 ml – flask To 150 ml and 200 ml for each 500 ml – flask , the production of Polysaccharides was increased to (4.58 gm / l ) . On acidic analysis of polysaccharides that produced using thin layer chromatography technique ( TLC), we obtained only one patch resembling glucose as an essential component of polysaccharides . Also infrared light (IR) showed absorption band at (850 cm¬-1), and this band was considered as a Characteristic feature of polysaccharides type Alpha (α ). The estimation of the specific optical rotation showed a reflection of the value /α / 20 = ْ 30 which was also considered a characteristic feature of polysaccharides type alpha (α ) . The limited viscosity (Real) for the produced sugar was (η=1.5) . The reaction of polysaccharides with Iodine was negative .
Keywords
Production Condition; Local Isolates; Partial Identification

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