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A comparative study of Rose Bengal and indirect ELISA tests for detection of the brucella antibodies in sera of cows in some areas of Salah El-Din province | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 12, Issue 4, 2012, Pages 19-23 | ||
| Author | ||
| Idrees B. A. Al-abdaly | ||
| Abstract | ||
| This study was conducted to determine seroprevalence of brucellosis in non vaccinated cows in some areas of Salah EL-Din and to compare Rose Bengal and indirect ELISA tests for the detection of the brucella antibodies. A total of 88 serum samples were examined from different areas( Al-Shirgat :44 , Al-Alum ;15 , Tikrit ; 15 , Biji :14 ) representing 250 cows distributed in the different areas. Results of Rose Bengal test showed that the total seroprevalence of brucellosis was 11.36 ٪ and the highest rate14.28٪ in Biji, while the lowest was 11.36 ٪ in Tikrit area. Indirect ELISA test showed that the total percentage was 20.45٪, the highest 26.66٪ was reported in Al-Alum area and lowest 6.66٪ was reported in Tikrit area. The compatibility by Kappa value between the two tests was 0.5 indicating that the sensitivity of indirect ELISA test compared with Rose Bengal test in detection of brucella antibodies in cows serum . | ||
| Keywords | ||
| Rose Bengal and indirect ELISA tests; cows | ||
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