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A rapid and non enzymatic method for genomic DNA extraction from whole blood and some other mammalian tissues | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 12, Issue 4, 2012, Pages 165-169 | ||
| Author | ||
| Adnan F. N Al-azawy | ||
| Abstract | ||
| Although several methods have been exist for genomic DNA extraction from blood or animal tissues samples traditionally, most of these methods consume long time and using expensive chemicals such as proteinase K and toxic organic solvent such as phenol. On the other hand, there is no rapid, simple one method for the extraction of genomic DNA from blood and animal tissues samples in the same time. Since the objective of this study was to development easy modified method for DNA extraction from difference mammalian tissues such as fresh or frozen whole blood, kidney, liver, heart and muscles in the same time. The description modified method have many advantages, reducing the time, using inexpensive materials, no phenol, in addition to small amount of mammalian tissue is required 50-100 mg and 2 ml from whole blood. Genomic DNA were obtained high molecular weight and good quality, shown by agarose gel electrophoresis and spectrophtometric analysis. These results shown that the modified method is simple, fast, safe, most economical, resulting in a high molecular genomic DNA of good quality from several mammalian tissues and can be used in medical laboratories and research centers. | ||
| Keywords | ||
| DNA Extraction; blood; mammalian tissues | ||
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