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Protection Properties of He-Ne Laser (632.8 nm) Against UV-light (253.7 nm) On the Lymphocytes Blood Cellsand Its DNA | ||
| Journal of the Faculty of Medicine | ||
| Article 1, Volume 54, Issue 3, 2012, Pages 246-250 | ||
| Author | ||
| Wijdan F. AL-Siaidy | ||
| Abstract | ||
| Background: He-Ne laser (632.8 nm) pre-irradiation may lead to modulate the damaging effects of ionizing radiation and decreasing in radiation damage on cells, by the induction of antioxidant defence mechanisms and accelerated the repair. Objective: To study the protection properties of He-Ne laser (632.8 nm) pre-irradiation against UV light (253.7 nm) damage on human lymphocyte blood cells and its DNA. Subject & Methods: 27 samples were processed only for lymphocyte blood cells separation, while a 45 samples for the extracted DNA from lymphocyte. The total of 72 samples were employed to evaluate the influence of He-Ne laser pre- irradiation against UV-light. Results: The results showed that He-Ne laser (632.8 nm) pre-irradiation on human lymphocyte blood cells and its DNA leads to a decrease in the damage which caused by UV light (253.7 nm) irradiation. Conclusion:The magnitude of protection depends on the He-Ne laser pre-exposure doses. A higher survival percentage of lymphocyte blood cells and its DNA occurred with low He-Ne laser dose. Keywords:He-Ne laser (632.8 nm) irradiation, UV-light (253.7 nm) irradiation, lymphocyte blood cells and its DNA protection. | ||
| Keywords | ||
| Ne laser; irradiation; Light; lymphocyte blood cells and its DNA protection | ||
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