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New Technique use to differentiate between Pseudo and true coagulase Activity of Staphylococcus aureus isolated from Different infected Sites in Patients from Baghdad Hospitals | ||
| Al-Mustansiriyah Journal for Pharmaceutical Sciences | ||
| Article 1, Volume 11, Issue 1, 2012, Pages 163-176 | ||
| Author | ||
| Dr. Mohammed kadum Al-Araji | ||
| Abstract | ||
| Abstract: A total of 300 strains of Staphylococci isolated from various pathological sites of infection from different hospitals; these specimens derived from cases of human infections were tested for staphylocoagulase activity. Test systems employing normal citrated rabbit plasma and the same substrate supplemented with inhibitors of thrombin and proteolytic enzymes (but not influencing the staphylocoagulase activity) were used for testing suspensions of bacteria and cell-free culture supernatants. A total of 200 strains clotted normal rabbit plasma; however addition of Trasylol and heparine resulted in positive results in 100 strains, whereas plasma supplemented with Trasylol and hirudin was coagulated definitely by only 70 strains.It is postulated that proteolytic enzymes of staphylococci interfere with staphylocoagulase-induced clotting and may simulate | ||
| Keywords | ||
| Keyword; production correlated to sites of infections | ||
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