Growth and pathogenesis of Enteropathogenic E.coli as affected by bacteriocin produced and purified from Lactobacillus isolates with or without Quercus infectoria  ( Manjakani ) extract.

A. Abdul-Ratha, Hassan; F. Al-Jumaily, Essam; H. Raheema, Rana

	Growth and pathogenesis of Enteropathogenic E.coli as affected by bacteriocin produced and purified from Lactobacillus isolates with or without Quercus infectoria ( Manjakani ) extract.
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 1, Volume 36, Issue 0, 2012, Pages 1-7 PDF (0 K)
Authors
Hassan A. Abdul-Ratha; Essam F. Al-Jumaily; Rana H. Raheema
Abstract
The antimicrobial activity of Lactobacilli has been widely exploited for prevention of food –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially in children, because of bacteriocin activity and the importance of herbal drugs, hence this study was designed to evaluate the synergistic effect of plant extract and bacteriocin produced by Lactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli. 1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C. 2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min and then purified by two steps method including extraction with n-butanol followed by gel filtration chromatography on Sepharose 6B column. The results showed that the specific activity was 1600 AU/mg protein with 8 purification folds and 12% recovery yield. 3. The antibacterial activity of Quercus infectoria with concentration 300 mg/ml was showed highly antibacterial activity in vitro and in vivo. 4. The result showed synergistic effect of Plantaricin with Quercus infectoria extract after experimental infection that induced by orally dosing with Escherichia coli in vivo. A result of histopathological study was recorded recovery of tissue.

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