Histopathological Effects of Aqueous Extract of Oleander (Nerium oleander) Flower in Albino Male Mice

Jamal Majeed, Laheeb

	Histopathological Effects of Aqueous Extract of Oleander (Nerium oleander) Flower in Albino Male Mice
Al-Mustansiriyah Journal of Science
Article 1, Volume 23, Issue 1, 2012, Pages 29-38
Author
Laheeb Jamal Majeed
Abstract
The effects of oleander (Nerium oleander) flower aqueous extract on different organs of male albino mice (heart, lung, brain, kidney and liver) were studied. Four group of mice were used in this study each group contain four mice, group A were gulp with (11mg/kg body weight) as a single dose/day for four days, group B were gulp with (22mg/kg body weight) as a single dose/day for four days, group C were gulp with (33mg/kg body weight) as a single dose/day for four days, while the last group (group D) gulp with distilled water as a control group, and the animals was slaughter on day 5 for evaluation. The obtained organs were subjected to histological processing, and sections were stained with hematoxylin and eosin. A microscopical examination of kidney, dilatation and degeneration of bowman capsule at the second (22 mg/kg) and third doses (33 mg/kg) while no such changes were observed at the first dose (11 mg/kg). In addition to shrinkage of glomeruli at the dose (33 mg/kg) was apparent when compare with control group. Severe congestion in blood vessel of the lungs and edema around the esophagus were noticed at the dose (33 mg/kg), while there are no changes at the doses (22 mg /kg and 11mg/kg) and control group. Edema and a slight congestion in brain tissues were observed at the dose (33 mg/kg), while there are no changes at the doses (22 mg /kg and 11mg/kg) and control group. With respect to the liver, there was hydropic degeneration at the dose (33 mg/kg), and mononuclear cell infiltration in the portal spaces with scattered necrosis of hepatocytes were founds, while there are no changes at the doses (22 mg /kg and 11mg/kg) and control group. These effects were dose-dependent. Heart sections revealed congestion and hemorrhage, especially in the myocardium regions. Varying degrees of coagulative necrosis of cardiac muscle cells that were associated with infiltration of mononuclear inflammatory cells were also observed at the doses (22 mg /kg and 33mg/kg) while there are no changes at the dose (11mg/kg) when compare with control group.

Statistics Article View: 126 PDF Download: 47