New Developed Analytical-Chemiluminometric (Sensitized) Continuous  Flow Injection Analysis, for Determination of Ciprofloxacin;  Application to Pharmaceutical Preparation

M.A. Shakir Al-Hashimi, Issam; Shakir Turkie Al-Awadi, Nagam

	New Developed Analytical-Chemiluminometric (Sensitized) Continuous Flow Injection Analysis, for Determination of Ciprofloxacin; Application to Pharmaceutical Preparation
Journal of Al-Nahrain University - Science
Article 1, Volume 15, Issue 4, 2012, Pages 11-21
Authors
Issam M.A. Shakir Al-Hashimi; Nagam Shakir Turkie Al-Awadi
Abstract
A newly developed analytical method characterised by its speed and sensitivity for the determination of ciprofloxacin via the improvement of the chemiluminescence system for luminol-H2O2-OH--ciprofloxacin via continuous flow injection analysis. The method is based on the indirect chemiluminescence-reaction (Sensitized) via the improvement of the amount of photons released through the oxidation of luminol-H2O2-OH- in the presence of ciprofloxacin as a sensitizer for the reaction. Linear dynamic range for the chemiluminescence-emission vs. ciprofloxacin concentration was 0.01-1 mmol.L-1 while C.O.D was 96.64%. The L.O.Q was 203 ng/sample. L.O.D. (S/N=3) = 6.01ng/sample from the stepwise dilution for the minimum concentration of lowest concentration in the linear dynamic ranged of the calibration graph. R.S.D.% for n=10 was <1% for ciprofloxacin with concentration of 0.5 mmol.L-1. the method was applied successfully for the determination of ciprofloxacin in four pharmaceutical drugs. Using paired t-test it was shown that there were no significant difference between the two methods and on that basis the new method can be accepted as an alternative analytical method
Keywords
Chemiluminescence; Flow injection analysis; ciprofloxacin; fluoroquinolones

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