- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Diagnostic Study on Brucella melitensis isolated from Human in Wassit province | ||
| Al-Mustansiriyah Journal for Pharmaceutical Sciences | ||
| Article 1, Volume 12, Issue 2, 2012, Pages 8-24 | ||
| Authors | ||
| Basil A. Abbas; Haethem Q. Mohammed; Jalal A. Tofah | ||
| Abstract | ||
| Abstract: This study was performed to identify the extent of the infection with Brucellosis caused by Brucella melitensis in humans in Kut city through a period of nine months from September 2007 till May 2008. One hundred blood samples were collected from humans and all were suspected in having brucellosis while twenty blood samples were collected from humans and all were known in not being suspected in having brucellosis and has no history in having such disease according to the performed serological tests (Rose Bengal test and 2-Mercaptoethanol test). The recorded cases for the brucellosis suspected samples were 58 Cases (58%) that positive for Rose Bengal test which the acute phases. With the use of 2–Mercaptoethanol test, the results showed that the chronic phase positive cases reached up to 12 samples (20.68%). Blood sample from the infected cases used for the purpose of bacterial isolation. Later by using Castaneda biphase medium the results showed that out of 58, 8 (13.79%) isolates were positive for the test. This method has been characterized by its time shortness for isolation. A number of morphological and biochemical tests had been carried including the direct examination, Catalase, Oxidase , Urease, IMVIC test, inoculation into nitrate reduction test medium and the ability to grow in the bacteriostatic dyes medium under the presence of basic fuchsin dye with two concentrations 1:50000 and 1:100000. The results of the biotyping of isolates showed that 2 isolates out of 8 isolates (25%) were agglutinated with monospecific antisera for Brucella abortus (A) so it was belonging to the biotype 2. While the rest 6 isolates (75%) which were agglutinated simultaneously with monospacific antisera for Brucella abortus (A) and with monospecific antisera for Brucella melitensis (M) so it was belonging to the biotype 3. | ||
|
Statistics Article View: 123 PDF Download: 10 |
||