Estimation of humural immune response on the rabbits that immunizing with Hydatid cyst antigens by using IHAT and ElISA.

MSc,; M. Edan, Ekhlas; BSc,

	Estimation of humural immune response on the rabbits that immunizing with Hydatid cyst antigens by using IHAT and ElISA.
Journal of the Faculty of Medicine
Article 1, Volume 51, Issue 3, 2009, Pages 332-335
Authors
; Ekhlas M. Edan;
Abstract
Summary: Background: Hydatid disease also known as echinococcosis or hydatidosis , is caused by infection with larva (metacestoide) of tape worms of the genus Echinococcus. Materials and methods: Twelve Rabbits were immunized with three types of antigens (Hydatid cyst fluid antigen; Protoscoleces antigen and Germinal and Laminated layer antigen) according to a specific immunization program and we used indirect hemagglutination test( IHAT) and Enzyme Linked Immuno Sorbent Assay(ELISA) for detecting the anti-Echinococcus antigens in the serum of these animals. Results: The highest titter of antibodies were recorded in the Rabbit serum when use the first antigen (Hydatid cyst fluid) by using IHA method (1:64)after the end of immunization program while in ELISA method reached (1:128), The serum level for the rabbit serum that immunization with the second antigen(Protoscoleces) has reached (1:256) by using IHAT and (1:512)when use ELISA, The highest titter of third antigen (Germinal and Laminated layer)has reached (1:128) by using IHAT and (1:256) when we used ELISA method. Conclusion: The highest antibodies titer could be found when use the Protoscoleces antigen more than Cyst fluid and Germinal and Laminated layer antigen. Key words: Hydatid cyst, E.granulosus
Keywords
Hydatid cyst; granulosus

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