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Uptake of Uranium by Callus Cultures of Two Acacia Species | ||
| Rafidain Journal of Science | ||
| Article 3, Volume 24, Issue 1, 2013, Pages 31-43 PDF (0 K) | ||
| DOI: 10.33899/rjs.2013.67495 | ||
| Authors | ||
| Davey J; Godbold D; Riyad A. Fathi; Hana S. Al-Salih | ||
| Abstract | ||
| This study aimed to use callus cultures of Acacia trees as indicator for the validity of employing these trees in the phytoremediation programs that were used to manage the pollution with uranium in soil. Various concentrations of uranium (0.0, 50,100, 200, 500 and 1000mg.l-1) in the form of uranyl nitrate were added to MS medium that used for the initiation and growth of Acacia callus in the presence of 0.5 mg/l of each NAA and BA. For comparison, two species of Acacia were used in this study, A. albida and A. nilotica. Fresh and dry weight of callus was determined along three growth periods (30, 60 and 90 days). levels of uranium in callus cells were determined using TXRF analysis. The Results showed that fresh and dry weight of callus increased with the increase of uranium concentrations except that grown on MS with 1000 mg.l-1 of uranium in which inhibition in both fresh and dry weight of callus after three months of growth was recorded .Analysis of the cellular content of uranium using TXRF revealed that the maximum level obtained when callus grown on MS with 500mg.l-1 . | ||
| Keywords | ||
| Acacia; uranium; phytoremediation | ||
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