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Spectrophotometric and High Performance Liquid Chromatographic Methods for the Determination of Dapsone in a Pharmaceutical Preparation | ||
| Rafidain Journal of Science | ||
| Article 11, Volume 24, Issue 1, 2013, Pages 128-145 PDF (0 K) | ||
| DOI: 10.33899/rjs.2013.67587 | ||
| Author | ||
| Lamya A. Sarsam | ||
| Abstract | ||
| The detailed investigation of a sensitive and selective spectrophotometric method for the determination of dapsone; based on the oxidative coupling reaction of dapsone drug with pyrocatechol in the presence of potassium periodate in acidic medium to form an intense, stable, purple-red coloured water-soluble dye, which shows a maximum absorption at 509 nm. Beer's law obeyed over the range of 10-500 µg /25 ml, i.e,0.4-20 ppm with a molar absorptivity of 1.05×104l.mol-1.cm-1and Sandell's sensitivity index of 0.0235 µg.cm-2,a relative standard deviation of ±2.60 to ±1.05%; and limit of detection, (LOD) of 0.1735 µg.ml-1.; and (LOQ) of 0.5785µg.ml-1. And the method does not require temperature control or solvent extraction. HPLC technique has been also developed for the measurement of dapsone. The analysis was achieved on a C18 column using water and acetonitrile in the ratio of 50:50 (V:V) as a mobile phase at a flow rate equals to 0.5 ml / min. and the detection was done spectrophotometrically at 297 nm. A linear relationship is obeyed over the range 0.5-20 ppm with a relative standard deviation (RSD) ±1.53 to ±0.83% and relative error -0.3 to +1.1 %. Both methods were applied successfully to the assay of dapsone in pharmaceutical preparation (Tablet). | ||
| Keywords | ||
| Spectrophotometricy; high performance liquid chromatography; dapsone; Pyrocatechol; reagent | ||
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