INFLUENCE OF MEDIUM COMPONENTS AND EXPLANTS ON INITIATION MULTIPLICATION AND ROOTING OF Citrus aurantifolia IN VITRO

H. Taha, Fadia

	INFLUENCE OF MEDIUM COMPONENTS AND EXPLANTS ON INITIATION MULTIPLICATION AND ROOTING OF Citrus aurantifolia IN VITRO
The Iraqi Journal of Agricultural Science
Article 1, Volume 42, Issue 1, 2011, Pages 92-100
Author
Fadia H. Taha
Abstract
This study was conducted in the tissue culture labrotaries of the Department Horticulture – College of Agriculture / University of Baghdad for the period 2007 -2009, study the possibility of in vitro propagation Lemon tree Citrus aurantifolia by various plant parts shoot tips ; single nodes , internodes and cleared of trees trees in developing extremely Fruit Research Station / Latifiya experience of a global design complete randomization. . And Sterilized plant parts at 0.1 % Murcury Chlorid . It has been noticed from the results achieved at the stage evolution to respond to the differing growth in vitro and recorded the highest proportion opens in the single node growth regulators where the percentage reached 75% . In the multiplication stage has studied the effects of different concentrations BA 0, 0.5 , 1, 1.5, 2, 3, 4, 5 and 7 mg / l , and studied the effect of GA3 concentration 1mg/l in media of containing different concentration BA 0.5 ,1,1.5 ,2 mg/l on the number of branches formed and size from , where they were to achieve the highest rate of increase branches In MS medium is equipped with 1.5 mg / l BA + 1 mg / l GA3, which was 6.9 branch /explant, The highest rate achieved in the lengths that enabled in MS medium is equipped with 1 mg / l BA + 1 mg / L GA3 and reached 1.43 cm . At rooting stage studied the effect of 1 and 2 mg / l from IBA, NAA on the rooting percentage and number of roots formed and the size from where achieved the highest percentage of rooting of 60% in the 1 mg / l NAA added to MS medium with half strength salts, and the highest number of roots 0.8 root / branch and 0.98 cm in half-strength MS medium salts plus 1 mg / l NAA. From this it fllows that the concentration 1.5 mg/l BA + 1 mg/l GA3 the best in the multiplication of shoots , as well as the facus 1 mg/ l BA + 1 mg/l GA3 have achieved a higher rate to the length of shoots , with the highest percentage of rooting in the1 mg / l NAA.We recommend that you consider these plants that we have we have obtained the untreated control and monitoring their performance . In addition to the study of concentrations and other types of Auxins and Cytokinins to get to the other combinations used in the micropropagation of the tree lime in vitro.

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