The role of variable temperatures and LPS in vitro on

K.Zgair,; Ayaid,

	The role of variable temperatures and LPS in vitro on
Al-Mustansiriyah Journal of Science
Article 1, Volume 17, Issue 2, 2006, Pages 1-9
Authors
;
Abstract
The concentration of IL-8 produced by peripheral blood leukocytes (PBLs) has been measured in vitro by using ELISA. , these cells have been collected from healthy donors, and it's cultures exposed to variable temperatures, 35°C, 37°C, 40°C, in presence and absence of Lipopolysaccharide (LPS) (as stimulator for IL-8 production) at variable times , ( Zero , 20 h, 23h, 43h,and 45h ) .This study revealed that there is an inverse relation – ship between temperatures and IL-8 concentration in comparison with 37ºC as a control in the absence of LPS and relative different was found in presence of LPS. The high concentrations of IL-8 were found at 35ºC in presence and absence of LPS and this increase still at all times. At 37°C the same thing was found but the concentration of IL-8 were less than at 35ºC and the concentration of IL-8 still high something at all times. But at 40ºC the decrease of IL-8 concentration was detected in absence of LPS but in presence of LPS the sharp high of IL-8 concentration was found at 23h but after this time the concentration of IL-8 was decrease to low level. From this study we found related effect between 40 ºC and LPS. Therefore increasing of IL-8 at beginning is very important in chemoattractic of immune cells and stimulate of cellular immunity but if IL-8 is still high the inflammation will happen so the decreasing of IL-8 will prevent that therefore the 40 ºC and LPS is a very good state of immune response with little injury.

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