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Extraction, Purification and Characterization of a Lectin from Phaseolus vulgaris L. cv. White Seeds (White Kidney Bean) | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 9, Issue 4, 2012, Pages 925-935 | ||
| Authors | ||
| Yasser H. Jalil; Mohammed A. Jebor | ||
| Abstract | ||
| The purpose of the research was to study the purification and characterization of lectin from Phaseolus vulgaris L. cv. white seeds. The lectin was purified by sequence of steps , namely , first with ammonium sulfate precipitation followed by ion exchange (DEAE cellulose) and gel filtration (sephadex G-200) chromatography, and finally by polyacrylamide electrophoresis (PAGE). Single band was observed in native PAGE . The lectin was shown to have molecular weight of 33 kDa in SDS PAGE and about 35 kDa in gel filtration and purified about 9.01 fold to final specific activity of 64 titer/mg of protein. The hemagglutination activity of the lectin was stable within the pH range from 4-11 and temperature range from 0°-50°C. Chemical modification results indicate that lysine and tryptophan were crucial for the hemagglutination activity of lectin. The results of carbohydrates specificity showed that the lectin was had complex sugar specifities, but not specific to xylose and mannose. | ||
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