Study The effect of Probiotic Prepared from Lactobacillus acidophilus on Adhesion of The bacteria Isolated from Dental Caries

	Study The effect of Probiotic Prepared from Lactobacillus acidophilus on Adhesion of The bacteria Isolated from Dental Caries
journal of kerbala university
Article 1, Volume 6, Issue 4, 2010, Pages 69-85 PDF (0 K)
Abstract
101 specimens were collected from patients with Dental Caries in Al-Diwaniya province from both sexes with different ages from February to May (2009). 152 isolates were rerecorded from both gram negative and positive bacteria. Results revealed the dominance of Streptococcus spp. 36.19 %, Staphylococcus spp. 27.69 %, Lactobacillus spp. 19.8 %. Species belong to Enterobacteriaceae were recorded 18.81 % and Pseudomonas aeuroginosa 4.60 %, Streptococcus mutans was the dominant 50.90 % from species of streptococci, while Staphylococcus epidermidis recorded the higher ratio 66.66% from species of staphylococci, In case of lactobacilli, Lactobacillus acidophilus recorded the high ratio 65.51% from isolated lactobacilli species, Escherichia coli was the dominant ratio 9.90 % of Enterobacteriaceae species. The recorded results showed resistance of Gram positive species to the most antibiotics in this study, specially to ampicillin 91.44 % and erythromycin 89.26 %, while were sensitive to refampicin 17.46 %, ciprofloxacin 22.22 % and tetracycline 35.71 %. while Gram negative species were resistant to ampicillin 88.46 % and amoxicillin 86.26 %, and sensitive to ciprofloxacin, tetracycline and gentamicin 15.38 % , 34.16 % and 42.30 respectively. The results explained existence of significant effects of three types of Probiotics that were got from L. acidophilus on adhesion of bacteria on epithelial cells and polymer discs of Light cure, the filtrate of cells gave the highest effect by reducing the numbers of adhered bacteria on epithelial cells of species S.sanguis, Staph. aureus, and Shigella spp. with numbers 17,23 and 19 bacteriacell respectively, while heat killed cells have high effects on S.salivarius, Staph. aureus and Shigella spp. with 11,13 and 14 bacteriacell respectively, the ultrasonic destroyed cells had effects on S .mutans, Staph. aureus, and Shigella spp. 23, 27 and 24 bacteriacell respectively. Similar results were seen in case of polymer discs, the effect of filtrate on S. salivarius, Staph. aureus and E .coli was high with value 4.8, 7.1and 4.7 cellmm2 respectively, while heat killed cells has a significant effect on S.salivarius, S.aureus and E.coli species with numbers 5.9, 7.6 and 6.1 cellmm2 respectively, the ultrasonic prepared probiotic had effect on S.sanguis, Staph. aureus and E .coli species with numbers 5.0, 4.5 and 5.3 cellmm2 respectively. The results of this study revealed that the sensitivity to antibiotic refampicin, ciprofloxacin and tetracycline when impregnanted polymer discs with antibiotic to test the adhesion of bacteria on these discs, while the resistance to the other antibiotics appeared in different ratios, the species of Gram negative were sensitive to refampicin and ciprofloxacin with less effect to tetracycline and high resistant to amoxillin and with less effect to the other antibiotic that used in this study.

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