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ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE | ||
| Basrah Journal of Veterinary Research | ||
| Article 1, Volume 11, Issue 2, 2012, Pages 145-158 PDF (0 K) | ||
| DOI: 10.33762/bvetr.2012.68925 | ||
| Author | ||
| Adnan .M . AL-Rodhan Hanaa K. Ibrahim | ||
| Abstract | ||
| Two hundred samples (cow’s and goat’s milk75 for each,their farm soil 25and 25water samples )from different origin have been cultured on Ashdown selective agar which gave a higher percentage (36.5%)for B. pseudomallei isolation , 64% was observed in farm soil followed by water samples 48% than cow’s milk33.33% and finally the goat’s milk 26.66%. According to L-arabinose biotyping , 39.72% of the B.pseudomallei isolates were positive for arabinose (Arb+) and 60.3% of isolates were negative for arabinose (Arb-).The virulence testing revealed that most bacterial isolates yield haemolysin, protease, lipase and lecithenase in a percentage of 97.3% , 82.2%, 89.04% and 84.9% respectively.The Arb- biotypes were more virulent than Arb+ biotypes with significant differences (P< 0.05).All bacterial isolates were characterized by their ability to form capsule(100%),.This study confirmed the presence of casein proteolytic and necrotizing enzymes in the suspension of B.pseudomallei .Proteins concentration in this suspension was 0.00248 mg/ml . | ||
| Keywords | ||
| Goat milk; Arabinose; Soil | ||
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