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Cytotoxic effect of esculetin on myeloma and myelogenous leukemic cells. | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 5, Issue 2, 2012, Pages 147-152 | ||
| Authors | ||
| Noor Al-Huda Ali A.H. Saeed; Khedhir Hassan Ali Al-Jorany | ||
| Abstract | ||
| The cytotoxic effect of Esculetin to mouse myeloma and human acute myelogenous leukemia cells invitro was evaluated. Tumor cells exposed to Esculetin in culture showed an exponential cytotoxic responses. A decreased viabilities of both leukemic cells were noticed with 20, 40 and 80 µg / ml Esculetin. Besides, myeloma cells showed a progressive depletion of viability when exposed continuously for 1,2,3 and 4 days, whereas , pretreatment of myeloma cells with Esculetin for 1,2,4 and 24 h followed by removal of the chemical and culturing the cells for 3 days indicated that the time the cells much effected was 24 h post exposure. At this exposure time , exposure to 10 µg / ml decreased the able cell number to 24.66 × 10-4 cells / ml versus 62.66 × 10-4 cells / ml in the control . t – test = 5.16 , p = 0.007 95 % CI = 17.5 – 58.5. Like wise , a 20 µg / ml concentration of esculetin decreased viable cells number to 23.5 × 10-4 cells / ml versus 62.66 × 10-4 cells / ml of control. T-test 7.39 , P = 0.002. 95 % CI = 24.5 – 53.8. The efficiency of Esculetin in protecting mice implanted with syngeneic myeloma was studied . The results of invivo experiment demonstrated that the chemical could protect mice from lethal dose of the experimentally implanted tumor . The protection value reached 66.7 % in animals treated with 200 or 400 µg Esculetin for 10 consecutive days. For 200µg drug plus myeloma versus myeloma only , chi – square value was 3.086 p=0.08 and 95% CI = 0-2.4. However , at 400 µg drug plus myeloma versus myeloma only , the chi – square analysis showed a value of 4.17 , P=0.04 95% CI = 0.02 – 1.3. Besides , the survival of the animals was increased . The therapeutic relevance and speculated mechanism of Escletin cytotoxic effects are discussed. In conclusion , it seems that esculetin has antiproliferative effects to mouse myeloma and to human myelogenous leukemia cells. | ||
| Keywords | ||
| Esculetin; Anti; tumor; Myeloma; Myelogenous leukemia | ||
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