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Study the Effect of Lactobacillus acidophilus Local isolate and its Components as colon anticancer induced in rats | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 5, Issue 2, 2012, Pages 183-190 | ||
| Authors | ||
| Nahi Y.Yaseen; Zahra M.Al-Khafji; Maareb N.Rasheed | ||
| Abstract | ||
| Knowledge of microbiology and its relation to tumor is in continuous progress.Many efforts directed to eradicate the tumor cell without hart the normal living body cells. The aim of this study was to investigate the role of Lactobacilli as a Probiotic in colon cancer treatment in rats. Colon cancer was induced in rats using Azoxymethane (AOM) at 15mg/Kg( animal body weight) by adding methyl group to guanine (O6- methyl guanine) . The later showed by using methylation sensitive restriction enzymes such as HpaII (sensitive to presence of methyl group ), and MspI (resistant to methyl group) so the samples treated with former will give a clear band of methylated DNA in gel electrophoresis while MspI will give diffused band when the DNA is methylated . The post- treatment effect of Lb. acidophilus and its components was studied as well .The animals were injected with AOM at one week interval for two weeks ,Then fed Lb . acidophilus and its components for 6 weeks started a week after the last injection. It has been shown that Lb .acidophilus cells reduced the ACF by 88.8%(only 9 foci were found compared to 80 foci in control treatment) the animal administrated with glycoprotein samples reduced the development of foci by 87.57%.(only 10 foci were found compared to 80 foci in control treatment). the animal administrated with cytoplasm sample and crude growth medium there was 86.25% reduction (only 11 foci were found compared to 80 foci in control treatment).either in terms of DNA samples extracted from the colon of rats (aberrant crypt foci) injected carcinogens and treatment cells and bacteria components Lb .acidophilus which explained after amonth and a half from the date of the second injection carcinogens, which when incubation with restriction enzyme HpaII methylation sensitive DNA enzyme , appeared smear wtthin the gel electrophoresis which enhances the role of cells and bacteria components Lb. acidophilus in the modulate DNA methylation compared with DNA samples extracted from the colon of rats positive group injected carcinogens which explained after amonth and ahalf later than the second injection of carcinogens and that when incubation with restriction enzyme HpaII appeared in broad and radiant within gel electrophoresis which indicates the presence of methyl group in the DNA of this animals group. | ||
| Keywords | ||
| Azoxymethane | ||
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