The protective role of grape seeds oil on damage induced by iron overload on thyroid gland in male rabbite

	The protective role of grape seeds oil on damage induced by iron overload on thyroid gland in male rabbite
journal of kerbala university
Article 1, Volume 7, Issue 2, 2011, Pages 264-273 PDF (0 K)
Abstract
This study was carried out to investigate the protective role of black grape seeds oil Vitisviniferaon thyroid damage induced by iron overload in male rabbits . Twenty five adult male rabbits were divided into five groups (5/group) , the first group was injected with 20 ml/kg distal water and served as control group (G1) . Rabbits in the second group were injected with 20 mg/kg iron dextran with one dose in the first week , two in the second week , three in the third week and four dose in the fourth week (G2). Rabbits in the third group (G3) were inject with 20 mg/kg iron dextran and intubated orally and daily with ED50 of black grape seeds oil which equal to 0.5 ml/kg, while the rabbits in the fourth group (G4) were injected 20 mg/kg iron dextran with SC injection 10 mg/kg desferrioxamine at one dose in the first week , two in the second week , three in the third week and four dose in the fourth week for each one . Rabbits of the last group were injected 20 mg/kg iron dextran, 10 mg/kg desferrioxamine and 0.5 ml/kg black seeds oil. Fasting blood samples were collected at pretreated , after two weeks and at the end of experiment to study the following parameters : the levels of T3, T4 and TSH hormones The results revealed intramuscular injection of iron dextran caused significant decrease (p<0.05) in levels of T3 and T4 hormones ,and significant increase (p<0.05) in levels of TSH hormone Comparative with control group . The group that treated with black grape seeds oil and exposure to iron overload by iron dextran revealed no significant difference was observed in T3, T4 and TSH hormones. The group that was exposed to injection with desferrioxamine revealedsignificant decrease (p<0.05) in T3 and T4 hormones , and significant increase (p<0.05) in concentrations of TSHhormone . Histological section revealed that iron overload caused significant decrease of colloid in thyroid gland, while the results showed normal feature of in thyroid tissue after oral gavages of grape seeds oil comparative with control group, and decrease of colloid after injection of desferrioxamine. In conclusion , results of this study confirm the protective role of black grape seeds oil against deleterious effect of iron overload in thyroid gland , and documented the prevalence of black grape seeds oil up on desferrioxamine

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