EFFECT OF DIFFERENT CONCENTRATIONS OF BA AND SUCROSE ON CALLUS INDUCTION AND INITIATION OF ASEXUAL EMBRYOS IN ORANGE (CITRUS SINENSIS L. OSBECK) NUCELLAR SEED TISSUES IN VITRO

M. Ibrahim, Kadhim

	EFFECT OF DIFFERENT CONCENTRATIONS OF BA AND SUCROSE ON CALLUS INDUCTION AND INITIATION OF ASEXUAL EMBRYOS IN ORANGE (CITRUS SINENSIS L. OSBECK) NUCELLAR SEED TISSUES IN VITRO
Iraqi Journal of Biotechnology
Article 1, Volume 11, Issue 2, 2012, Pages 196-208
Author
Kadhim M. Ibrahim
Abstract
Plant tissue culture techniques were exploited for the micropropagation of sweet orange (Citrus sinensis L. Osbeck) seed nucellar tissues as a source for raising asexual embryos and thereafter plants. Callus was induced from the nucellar tissues and subsequent induction of somatic embryos. Callus fresh weight, number of asexual embryos, shoots and their length and root number were investigated. Sucrose concentrations (2, 4, and 6 g/L) and Benzyl adenine (BA) at 0.0, 0.5, 1.0 and 2.0% were added to Murashiege and Skoog (MS) medium.The addition of 0.5 mg/l of BA in the presence of 2% sucrose to the medium caused highest callus fresh weight (295mg), while supplementation with 1.0 or 2.0 mg/l BA in the presence of 4% sucrose resulted in highest number of somatic embryos per exiced tissue from single seed seed. Supplementation of the medium with 1.0 or 2.0 mg/l of BA and 6% sucrose recorded the highest number of shoots per single tissues. Shoots rasied from callus tissues were rooted on the same medium but containing 0.0 or 0.5 mg/l BA in the presence of 4% sucrose achieving the highest mean number reached 2.5 roots/shoot. Successful acclimatization of plantlets was achieved when they transferred to a mixture of peat moss and riversand media. Acclamatizsed plantlets were transferred successfully to pots with a survival percentage of 80%.
Keywords
Citrus sinensis; Asexual embryos; Callus Induction; Nucellar tissues; Plant growth regulators; Sucrose levels

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