Immunohistochemical study of BCL-2, PCNA and VIMENTIN markers in oral and laryngeal squamous cell carcinoma (Comparative study)

F. Muhsin, Shaimaa; A. Alsodani, Kadhim

	Immunohistochemical study of BCL-2, PCNA and VIMENTIN markers in oral and laryngeal squamous cell carcinoma (Comparative study)
Journal of baghdad college of dentistry
Article 1, Volume 23, Issue 0, 2011, Pages 82-86
Authors
Shaimaa F. Muhsin; Kadhim A. Alsodani
Abstract
Background: Squamous cell carcinoma (SCC) may arise in any stratified squamous epithelium or muosa that has undergone squamous metaplasia. The aims of the study were immunohistochemical evaluation and comparison of PCNA, BCL-2 and VIMENTIN expressions in oral and laryngeal squamous cell carcinoma and correlating such expressions with the clinicopathological behavior in both sites. Materials and Methods: This study was performed on thirty formalin-fixed, paraffin-embedded pathologically diagnosed oral and laryngeal SCC blocks for the period of June 2006 till July 2010.Age, sex, site and histologic grades were recognized. The samples were immunohistochemically stained with monoclonal antibodies toBCL-2, PCNA and VIMENTIN. Results: The age of the patients with squamous cell carcinoma of the oral cavity was between 40 and 86 and between 32 and 66 years in larynx. The male/female ratio was 10/5 and 8/7 for oral and Larynx respectively. The majority of the cases of Oral squamous cell carcinoma were located on the buccal mucosa, Whereas Laryngeal squamous cell carcinoma cases were distributed on glottic mainly..No significant statistical difference in the age and gender between the two groups .Histological grading was recognized for each case of oral and laryngeal squamous cell carcinoma. Two thirds of the oral cases were well differentiated. While 8 of the fifteen cases were moderately differentiated SCC. Regarding histological grading there was no statistically significant difference between the two groups .PCNA expressed in 14 of the fifteen oral cases whereas in laryngeal, 12 cases were positive. Collectively, 9 of the 30 cases were positive for vimentin antibody with different score values,.Bcl-2 was expressed in one case only from fifteen immunostaining laryngeal cases and all the oral squamous cell carcinoma cases were negative No significant relationship in immunoexpression of he above markers between the tow groups. Conclusions: The results of this study proved that the biological behavior namely the PCNA, VIMENTIN and BCL-2 activities was comparable between SCC of the oral cavity and larynx with a spectrum of clinical behavior, due to the differences in location
Keywords
Squamous cell carcinoma of the oral cavity and larynx; PCNA; BCL; Vimentin

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