In vetro effect of Gibberellin and cultivar on induction and         sprouting microtuberization potatoes cv. Diamant,  Desiree,  and Marfona

	In vetro effect of Gibberellin and cultivar on induction and sprouting microtuberization potatoes cv. Diamant, Desiree, and Marfona
journal of kerbala university
Article 1, Volume 6, Issue 2, 2010, Pages 34-44 PDF (0 K)
Abstract
This stady was conduted during the period 2009 / 2008 in the tissue culture lab. Biotechnology Department- Technical College / Musaib. Sprouts of Diamant, Desiree and Marfona potato tubers ( 0.3 – 0.5 mm) were transferred on the propagation medium. Explants were sterilized with sodium hypochlorite at 5% for 15 min., cultures were incubated at 25º C ± 2 and 16 hours photoperiod at light intensity 1000 lux for 35 days. The (1 – 2 cm.) basal parts of the producing cuttings were grown on microtuberization MS media containing 8% sucrose plus 2.0 g / l charcoal. The first study contained treating the microtubers with Capitan at 2g / 1kg tubers to study the effect of cultivar and size on the percentage of the damaged tubers, sprouted tubres, number of developed buds/ tuber and vegetative buds / tuber, before and after the storage. The second trial was about the effect of cultivar and Gibberellin ( 0.0, 3.0, 4.0, 5.0 and 6.0 mg / l ) in the characters studied. The interaction between cultivar and size had a significant influence. Hightest results were obtained from Desiree with the larger size S1 regards bud number / tuber, number of grown buds, ( 6.97, 5.31 ) respectively, wheras, Diamant with S1 resulted in less damaged tubers and higher sprouting ( 4.05%, 99.29% ) respectively. Interaction of Desiree with GA3 at 5.0 mg / l had significant effect on number of developed buds ( 5.31), while Diamant with 5 mg / l Gibberellin gave higher sprouting ( 89.14% ) on the other hand there was a decrease in dry metter for all cultivars.

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