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STUDY THE GENOTOXICITY EFFECT OF HOECHST 33258 STAIN IN THE HUMAN LYMPHOCYTE | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 2, 2012, Pages 209-218 | ||
| Authors | ||
| ; Muhammad A. Al-A | ||
| Abstract | ||
| Most stains using in DNA labeling has the ability to cause genetic mutations because of its ability to intercalated on DNA. Hoechst 33258 stain is one of a widely used stains in laboratory preparations, some studies indicated that this stain caused genetic mutations. It is important to detect the relationship between this stain and the people who works in laboratories, there is a lack of studies describing accurately the genotoxicity of this stain. This study is an investigation on the genotoxicity of Hoechst 33258 stain in human lymphocytes (in vitro) using cytogenetic tests including Mitotic Index (MI), Chromosome Aberration (CA), Sister chromatid exchange (SCE) and Preparation of micronuclei, using different concentration of Hoechst stain 5μg/ml, 10μg/ml, 15μg/ml, 20μg/ml. The results showed significant increase in Mitotic Index (MI), Chromosomal Aberration (CA), Sister chromatid exchange (SCE) and Micronuclei this increase is direct proportion with the increase of stain concentration. The Chromosome Aberration represented by chromosomal and chromatid breaks showed at (5,10,15,20)μg/ml, chromosomal and chromatid gap showed at (5,10,15,20) μg /ml, while ring chromosome and dicentric chromosome showed at (15,20) μg /ml. | ||
| Keywords | ||
| genotoxicity; Hoechst; lymphocyte | ||
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