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DETECTION OF SALINITY TOLERANCE CAPABILITY IN SOME GENOTYPES OF SUGAR BEET BASED ON PCR TECHNIQUE | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 44, Issue 2, 2013, Pages 194-199 | ||
| Author | ||
| Majid Sh. Hamdalla | ||
| Abstract | ||
| DNA was extracted from eight cultivars of Sugar beet (Beta vulgars), six of them are grown in Michigan state(EL-AO 13698, EL-AO 21483, EL-AO 22799, EL-AO 22438, EL-AO 15037, EL-AO 15028) one is wild and the last is introduced with five primers for five loci of salinity tolerance which are imported from Integrated DNA Technology company at the laboratories of the College of Plant and Soil Science/Michigan State University. The objective was to identify some salinity tolerance genes and estimate the genetic diversity based on PCR technique for the tested genotypes. Genetic diversity was estimated and cluster analysis was applied based on agglomerative method which accumulated the tested genotypes into groups according to resemblance of their responses. Twenty seven polymorphic bands were produced. The rate of bands was 4-7 for each genotype and maximum bands were produced by primer BVU07582-m and BVU07582-U. The tested genotypes grouped in three sets according to their alleles, the group A includes the genotypes EL-AO 22799, EL-AO 22438,and EL-AO 15028, group B includes the genotypes EL-AO 13698, EL-AO 21483, EL-AO 15037, and Swiss, while group C contain the genotype Wild. The genotype Wild was the most diversity, while the genetic diversity for group A was equal and faint and gave bands for all used primers. This may explain the responses similarity of genotypes of A group for the used primers, while no bands were formed for genotype Wild except primer F100-p49296-L. The genotypes EL-AO 22799, EL-AO 22438, and EL-AO 15028 have all the sequences of primers which code for salinity genes. | ||
| Keywords | ||
| Sugar Beet; Salinity tolerance genes; PCR | ||
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