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THE INHIBITION ACTIVITY OF AQUEOUS EXTRACT OF PEGANUM HARMALA SEEDS IN GROWTH OF SOME PATHOGENIC BACTERIA | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 44, Issue 2, 2013, Pages 234-240 | ||
| Authors | ||
| Moayed A. Touej; Suhaila Ghafory; Amir K.Abbas; Gulboy A. Nasir; Abdul Jabbar A. Al-Khazraji | ||
| Abstract | ||
| An experiment was conducted to evaluate the inhibition activity of different concentrations of aqueous extract of Peganum harmala L seeds extract (25-500 mg/ml) in growth of some pathogenic G-bacteria species (e.g. E.coli, Pseudomonas aeruginosa and Salmonella spp.( and G+ species (e.g. Staphylococcus aureus and Bacillus spp.). Activity of Peganum harmala seed aqueous extract in bacterial inhibition was tested by well-diffusion agar with piercing cork (8mm diameter).Result revealed, that different concentrations of aqueous peganum harmala L seeds extract was exhibited high inhibition activity in growth of G+ bacteria species in the comparison of G- bacteria. The inhibition zone diameter at 25mg/ml conc. of aqueous seed extract was 14 and 12 mm for G+ Bacillus spp.and Staphylococcus aureus respectively in the comparison with G- species (E.coli, Pseudomonas aeruginosa and Salmonella spp.( which was 7,Nil and 6 mm respectively, similar trend to that of 50 mg/ml conc. was found, inhibition zone was 15 and 18 mm for Bacillus spp. and Staphylococcus aureus respectively, whereas it was 9,Nil and 10 mm for E.coli, Pseudomonas aeruginosa and Salmonella spp. respectively. Inhibition zones were extrusive proportioning with increasing of concentration it was elevated positively with the extract concentration increasing for all species of pathogenic bacteria species which used in this study. The maximum inhibition zone diameters was 13 mm and 27mm for E.coli and Staphylococcus aureus at 300 mg/ml conc., it was 15 and 20 mm for Salmonella spp. and Bacillus spp. respectively at 350 mg/ml conc., of aqueous pegnum harmala L seeds extract, last but not least the maximum inhibition zone diameter was 22mm for Pseudomonas aeruginosa at 400 mg/ml concentration. | ||
| Keywords | ||
| Peganum harmala; aqueous extract; antibacterial activity | ||
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