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Isolation and purification of Hepatotoxin (Microcystin-LR) from some Blue –green algae of sweage water in Basrah | ||
| Marsh Bulletin | ||
| Article 1, Volume 3, Issue 1, 2008, Pages 1-16 PDF (0 K) | ||
| Authors | ||
| M. J. AL-Aarajya; and E.Y.A. Al-Sultanb aCollege of | ||
| Abstract | ||
| The present study involved isolation, identification and purification of four species of Cyanobacteria Microcystis aeruginosa Kuetz , Microcystis flos-aque (Wittr.)Kirchner , Hapalosiphon Welwitschii (West and West ), Calothrix parietina (Thnret.) from Basrah region at AL-Ashar and AL- Khandaq canals that have been recorded for the first time in Iraq in this habitat .The extracted species testing for its ability to produced toxins particularly hepatotoxins (Microcystin–LR). This toxin was diagnosed depending on chemical features by using the thin layer chromatography technique (TLC). Toxic study showed that the toxin proceed by the algae has an (Rf) value closely to the standard hepatotoxin (Microcystin – LR) (0. 58). However, the value for the purified toxin was (0.54-0.56). The Ultraviolet spectrum of the purified toxin showed two absorption peaks ; the first appears at (240-243) nm and the other at (275-280) nm these two peaks are so close to the absorption peaks of the standard toxins ( Microcystin-LR), namely (239) nm for the first peak and (275) nm for the other. Whereas Infrared spectrum has shown that the purified toxin from the four species of toxic algae contains most of the active groups composing the structure of Hepatotoxins ( Microcystin-LR ) . By using the HPLC technique, the purified test has shown that the toxins have a retention time ranging between (3.920-4.108) minutes which is quite close to the retention time of the standard hepatotoxins Microcystin-LR (4.037) minutes. The results revealed that the species H. Welwitschii contains the highest concentration of hepatotoxin Microcystin – LR (44.415) μg/ml among the other species. The Bioassay tests for two species extracts of toxic algae have shown that the LD50 was within the toxic range of algae toxicity which reached (740) mg dry cells/kg mice for H.Welweitschii and (1540) mg dry cells/Kg mice for C. parietina, while the concentrations (20 and 50) mg dry cells/ml for all toxic species killed half numbers or more of the Artemia salina larvae after 24 hours | ||
| Keywords | ||
| Purification; Hepatotoxin; Microcystin; blue | ||
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