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Determination of Methionine Via Chemiluminescence-Continuous Flow Injection Analysis | ||
| karbala journal of pharmaceutical sciences | ||
| Article 1, Volume 4, Issue 4, 2013, Pages 190-204 PDF (0 K) | ||
| Authors | ||
| Issam M.A. Shakir; Nagam Shakir Turkey; Raad Faleh Hassan | ||
| Abstract | ||
| The research work represent a fast and simple method for the determination of methionine using chemiluminescence for the methionine-sodium hydroxide-luminol for the generation of a chemiluminesecent derivative of luminal. The emission was measured by continuous flow analysis made sample size of 83µL was used. Response versus concentration extended from 0.2-20 mM.L-1 with a percentage linearity of 96.17% or with 99.17% percentage of linearity for the range 0.6-20 mM.L-1. Reaching to a L.O.D. at (S/N=3) for 5 µM.L-1 from the gradual dilution for the minimum concentration in the calibration graph with a repeatability of less than 0.5% (n=10). A comparison was made between the new developed method with the classical method for the spectrophotometric determination by coupling with paired t-test or F-test and found that there is no significant difference between the two methods and the new adapted method which can be used as an alternative method. | ||
| Keywords | ||
| Methionine; chemiluminescence; continuous flow | ||
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