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Production of lipase from local isolated of Penicillium isolated from the seeds of nuts | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 18, Issue 1, 2013, Pages 40-47 | ||
| Author | ||
| Noor Amir Alubeidi | ||
| Abstract | ||
| Three local fungal isolates were isolated and identified from nuts that belong to Penicilliu waksmanii P. islandicum and P.rugulosum . The study was secreened the ability of fungal isolates to produce lipase using the method of solid media containing Tween -80 Tributyrin substance. The results showed that P. rugulosum was highest efficieny in excreting lipase. Therefor it was selected the plant oils are used as carbon source include (soy bean oil , olive oil and seasame oil). The enzyme activity reached to 20.56 U ml after 6 day when using seasame in medium. The best concentration of seamame in medium was 2 % UV ( 22.5U ml ). The enzyme activity reached to 27 U ml after adding urea to the production medium as nitrogen source . | ||
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