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Effect of Gelatin Addition to Extender On Semen Quality of Rabbit | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 22, Issue 2, 2011, Pages 7-12 | ||
| Author | ||
| Sabah A. H. A. Rahman | ||
| Abstract | ||
| The effect of gelatin addition to extender on the viability and acrosome integrity of rabbit spermatozoa was studied. Pooled semen samples were processed in a tris based extender with two different levels of gelatin that solidify semen at 50C preventing sperm precipitation and unwanted energy expenditure movement. Semen was collected with an artificial vagina weekly from six rabbit bucks. Pooled semen was diluted in tris-based diluted which was provided with 1g or 1.8 gelatin (g/100ml extender). Dilution rate was 1 semen: 3 extender. The Final concentration rate was 80 – 100 X 106 spermatozoa /100 ml After storing semen up to 2 days at 50 C , semen samples were pre –warmed to 250C then evaluated with light microscope to reveal the influence of gelatin addition and preservation time (0 ,24 and 48 hrs) on sperm motility parameters. Adding gelatin to the extender improved motility and reduced dead and abnormal spermatozoa and acrosomat damage. The low gelatin content extender improved these parameters, but the high gelatin content extender gave the best results. However, increasing storage time resulted in reducing these parameter Results showed that gelatin addition had a significant positive effect on the quality of the stored semen which would facilitate commercial distribution. | ||
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