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A Novel Biochemical Study On Pectate Lyase Produced By Erwinia Chrysanthemi Isolated From Spoilt Potatoes Infected With Blackleg Disease | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 24, Issue 1, 2013, Pages 23-36 | ||
| Author | ||
| Sahira N. Muslim | ||
| Abstract | ||
| Out of 25 spoilt potatoes samples from local markets in Baghdad city. Six isolates from Erwinia chrysanthemi were obtained(24%). The isolate that gave higher pectate lyase activity was choised to purify pectate lyase through two stages of purification including (ethanol precipitation and gel filtration by Sephacryl S-100) with 35.2- fold purification, 60 U mg-1 specific activity and 36% yield. The molecular weight of the enzyme was 42 kDa by gel filtration chromatography. The maximum activity of the enzyme was found after 25 min incubation at 50°C and pH 8.5. The addition of phenolic acids(0.05 mM) and thiols(1 mM) had inhibitory effect on the enzyme activity except L-cystine that showed an increase in the enzyme activity to 115%. The enzyme revealed maximum activity in the presence of polygalacturonic acid (0.1% w/v) as substrate at pH 8.5 and 50°C. I | ||
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