Numerical analysis of electrophortic protein patterns of Pseudomonas aeruginosa and Pseudomonas fluorescens/ putida group isolated from patients in Baghdad

Abdul Hamid Said, Layla

	Numerical analysis of electrophortic protein patterns of Pseudomonas aeruginosa and Pseudomonas fluorescens/ putida group isolated from patients in Baghdad
Al-Mustansiriyah Journal of Science
Article 1, Volume 24, Issue 1, 2013, Pages 61-76
Author
Layla Abdul Hamid Said
Abstract
Whole cell protein patterns obtained from 14 isolates of Pseudomonas aeruginosa (pa) and 6 isolates of Pseudomonas fluorescens/ putida (pf/p) group using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were analyzed. The protein bands and its percentage were determined according to the schematic diagram obtained by special software (Photo Molecular Weigh Software, 200 Version 10.01). The SDS-PAGE technique generated 12 different protein profile patterns; the individual isolate had 20 to 26 protein bands ranging in their molecular weight from 406 to 14 KDa. The resulting electrophoretic protein patterns obtained were used as bases for numerical taxonomy based on Jaccard's coefficient (Sj) and Unweighted Pair Method using Arithmetic Averages (UPGMA). Cluster analysis of the whole cell protein profile (WCPP)of Pa and Pf/p isolates obtained a dendrogram consisted of two major clusters that could be distinguished at Sj≥66.7% for both, only two isolates remains outside. After numerical analysis of the resulting electrophoretic protein patterns Pa and Pf/p isolates grouped together with Sj reaches >100% in the two clusters which indicate the close relationship among these isolates.

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