- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
LECTIN HISTOCHEMISTRY OF TRACHEO-ESOPHAGEAL REGION IN CHICK EMBRYOS | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 11, Issue 1, 2013, Pages 4-11 | ||
| Authors | ||
| Hayder J Mubarak; Amal A. Al-Taee; Ali Sh. Al-Araji | ||
| Abstract | ||
| Background:Glycosylation is an important modification involved during embryonic development. Lectins are specific carbohydrate-binding proteins; they can be employed as specific probes to localize defined monosaccharide and oligosaccharides on cell surface and on cytoplasmic structures, and in extracellular matrix. Objectives:The lectins (SBA, PNA, WGA, SWGA, UEA-I) binding were used as a sensitive, stable, and easy tool that can provide an extraordinarily sensitive detection for changes glycosylation and carbohydrate expression that may occur during embryogenesis and development of trachea-esophageal region. Methods:Fertilized chick eggs were incubated at 38 °C, embryos were fixed with Bouin’s solution. Sections were treated with fluoresce ineisothiocyanate (FITC) labeledlectins. Results:The histochemical study during the 2ndand 3rddays of development revealed variable tempo-spatial variability of lectin bindings to the mesenchymal tissues and other embryonic structures at the trachea-esophageal region. Conclusions:The lectin bindings could be an indicator for the glycoconjucate changes that play an essential role in developmental phenomenon of trachea-esophageal morphogenesis by marking cellular differentiation, cellular migration, and cellular interactions. Key words:Trachea, esophagus, chick, embryo, lectin, histochemistry. | ||
| Keywords | ||
| Trachea; esophagus; chick; Embryo; lectin; Histochemistry | ||
|
Statistics Article View: 144 PDF Download: 92 |
||