Association of HLA -DQB1*0305 with Type I Hypersensitivity in beef allergic patients

And  Fawziah  Ali Abdulla, AaJabbarRaisan; Shym,

	Association of HLA -DQB1*0305 with Type I Hypersensitivity in beef allergic patients
Journal of Basrah Researches (Sciences)
Article 1, Volume 39, Issue 1, 2013, Pages 47-57 PDF (0 K)
Authors
aaJabbarRaisan and Fawziah Ali Abdulla;
Abstract
Food allergies are important health problems. Since there are no previous studies to assess food allergies to beef in Iraq. So the aim of this study reveals food allergies by using immunological and genetic testing . Two hundred and sixty four sera from suspected meat allergic subjects were tested by in direct ELISA for specific IgE antibodies to beef .Polymerase chain reaction technique was performed to detect genetic relationship between food allergy and human leukocyte antigen classII allele(HLA -DQB10305). Depending on the ELISA seropositivity results, 13.3% of allergic patients were seropositve against beef allergens raw and cooked and the higher rate of seropositivity was observed in males (14.6%) and in 2nd age group patients (17.9 % ) in concern to sex and age effect on the seropositivity.There were non significant (p>0.05) variable values of optical density mean and standard deviation. The loss of beef allergy was not observed in all allergic patients and all of them showed the same positive raw and cooked beef specific IgE response .The results of PCR amplification showed successful binding between the specific primers of HLA- DQB10305 and the extracted DNA appeared as single band of expected size, 195bp . The positive specific IgE response to beef allergens was significantly associated with HLA DQB1*0305 .
Keywords
Beef meat; HLA class II; HLA; DQB

Statistics Article View: 127 PDF Download: 55