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Extraction , Purification and Characterization of alpha- amylase from germinated Sorghum seeds | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 39, Issue 1, 2013, Pages 13-26 PDF (0 K) | ||
| Author | ||
| Dhia falih alfekaiki | ||
| Abstract | ||
| The study included germination of sorghum cereal and alpha- amylase was followed up during the germination stages, the results showed increase in the activity of amylase in five day from germination 1600 U/ml .after that was selected of the best methods to extract the enzyme among fore methods. The purification stages were done by two steps included precipitation by ammonium sulphate at (30-70) % saturation and gel filtration on sephadex G100. The final purification folds and the yield of the enzyme were 19.31. times and 14.60%, respectively. The purified enzyme showed following characteristics: The optimum pH of the enzyme activity was 6. The enzyme was most stable at pH (4.5 - 6). The optimum temperature for its activity was 65 c. The enzyme retained its original activity when incubates at (30-70) c for 20 minutes. | ||
| Keywords | ||
| Key Word; Alpha amylase; sorghum germination | ||
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