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Influence The Effectiveness of Compounds Produced From Algae Oscllatoria Princeps Agains t Bacteria and Detected of Some Fatty Acids | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 54, Issue 2, 2013, Pages 266-273 | ||
| Authors | ||
| dauu Shaukat; Ghaidaa H. Al.Rubaiee | ||
| Abstract | ||
| In this study Oscillatoria princeps were isolated ،purified and identification from water canal in Almustansiriah University . BG-11 culture media was used for their cultivation in suitable laboratory conditions (25c°, 200µE/m2/sec) for 16:8 hrs. Light: dark. Each culture was harvested at the end of exponential phase .Organic solvents used for extraction was Chloroform: methanol at 2:1 to extract the crude active Intracellular and Extracellular substances, and evaporated down to dryness. Antibacterial activity of these different extracts were evaluated against 10 strains of gram positive bacteria and gram negative bacteria, Agar diffusion method was used in this evaluation. Results showed that the intracellular products which extracted beast than extracellular product. The gram positive bacteria studied revealed higher susceptibility to attack by the intracellular and extracellular extracts comparing with the gram negative bacteria. The intercellular extraction revealed higher antibacterial activity against Staphylococcus arueus average of inhibition zone was 30mm. but The extracellular extraction revealed higher antibacterial activity against Bacillus subtilise average of inhibition zone was 28 mm. However, Results showed gram negative bacteria intracellular products extract has the antagonistic activity against Pseudomonas aeruginosas with 25 mm inhibition zone. also been detected on some fatty acids produced by the algae O.princeps which Palmitic , Stearic, Arachidic , Linoleic and Linolenic . the highest rate is fatty acid Palmitic 75% of the extract intercellular while the rate of 34% of the acid fatty Linolenic to extracellular abroad. | ||
| Keywords | ||
| blue; algae extract; antimicrobial; inhibition | ||
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