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Plasmid Role in Crystals Protein Production in Locally Isolated Bacillus Thuringiensis | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 54, Issue 2, 2013, Pages 301-306 | ||
| Authors | ||
| Mohammad Adil Jaafar; Abdul Kareem Al-Kazaz; Ali Sadiq Mohammad | ||
| Abstract | ||
| The ability of crystals protein production was studied in local isolate Bacillus thuringiensis by microscope examination, and the crystals protein production were seen after 48 hour from growth starting, the study of plasmid profile of the isolate using gel electrophoresis technique in 0.7% of agaros gel, revealed that it had one Mega plasmid band, the plasmid role in production of crystals protein was studied by using conjugation experiments, that they applied on the local isolate B. thuringiensis which was crystals protein producer and Rifamycin sensitive as donor cells, with Bacillus spp. which didn’t produce crystals protein and Rifamycin resistance as recipient cells. Conjugation experiments assured the transmission of crystals production trait with Plasmid to the recipient cells, this result confirmed that crystals protein trait was Plasmid bourn. | ||
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