Surgical Embryo Transfer Method in the Native Iraqi Cows

M. Saleh, W.

	Surgical Embryo Transfer Method in the Native Iraqi Cows
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 5, Issue 2, 2012, Pages 57-62 PDF (178.27 K)
Author
W. M. Saleh
Abstract
Three virgin cows of 5-6 years old were selected as Donors of embryos, extensively, their genitalia was examined rectally, with a history of 1-2 normal pregnancy. After using PGF2α analogue (loprosteol), field observation for the normal cyclicity was conducted twice daily. The donors was super ovulated with a single injection of 3500 IU of PMSG at day 10 of the cycle (E=0), their ovaries was examined by next day rectally for the achievement of action of the hormone, donors were re-injected with PGF2αanalogue (loprosteol) on day 12 and inseminated as the signs of the cycle appeared. Embryos were collected non-surgically on day 6 after the insemination, examined and graded by stereomicroscope and directly transferred to synchronized recipient and follow them to term. 12 recipients of native breed, mainly SHARABI and GENOBI. Iraqi cows were selected; their genitalia were examined rectally and observed twice daily for normal cycle then synchronized with the donors by two injections of PGF2α analogue 11days apart. Both donors and recipients were already synchronized, embryos surgically transferred to the horn epsilateral to the ovary with corpus luteum. The results showed that 8 recipients stayed without signs of estrus until day 45 after transferring, one of them continued the term to parturition, 4 recipients showed estrus signs on days 24-36. The study showed that there is a good ability to apply this technique with a simple inquiries and facilities and easily attempt in field condition and on the native breed safely and can be used it as a tool of breeding performance.
Keywords
cows; embryo transfer

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