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Evaluate the Efficiency of Some Sterilization Methods in Reducing Expalnt’s Contamination for Date Palm (Phoenix dactylifera L.) | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 5, Issue 2, 2013, Pages 47-55 | ||
| Abstract | ||
| Abstract: This research was implemented to evaluate some methods for explants sterilization to reduce contamination for date palm cv. Bream. Four types of explants were used as an explants source: shoot tips, leaf primordia, axillary buds and mantel meriestems. Various concentrations of colorox (Soduim Hypochlorite) (0, 30, 40 and 50%) and potassium permanganate (KMn04) (0, 200, 300 and 400 mg/L were used for (10, 15, 20 or 25 min) under vacuum. Contamination percentage and Total Bacterial Count (TBC) were calculated. Results showed that treatment with 50% NaOCl combined with 300 mg/l KMnO4 for 20 min reduced contamination to 10% in axillary buds and mantle meristems as TBC recording 1.88, 0.92 log10 cfu respectively with 17.5% contamination for each. The TBC was zero in shoot tips and leaf primordia as bacterial colonies were completely disappeared. As a conclusion, we can adopt the above results as a surface sterilization method in the micropropagation protocol of date palm cv. Bream. | ||
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