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Preparation of New HPLC Stationary Phase and Study of Its Chromatographic Performance Toward the Separation of Some Water-Soluble Vitamins | ||
| Journal of Al-Nahrain University - Science | ||
| Article 1, Volume 16, Issue 2, 2013, Pages 51-58 | ||
| Authors | ||
| Saif S. Abdull-Sattar; Shahbaz A. Maki; Noor M. Ali | ||
| Abstract | ||
| A new stationary phase was prepared by the reaction of a crystal violet solution with silica gel. The capacity of the new prepared resin was calculated and the found average was 6.6meq./g. The resulted resin was highly rigid with high stability and used as stationary phase for HPLC column. Some water-soluble vitamin C, B2, B6, and B12 were examined by this column with isocratic eluention distilled water and methanol (2:98, (v/v)) as a mobile phase with flow rate of 0.5 ml/min and UV detection of 230 nm. The chromatographic performance of the packed column was characterized. The number of plate numbers (N), height equivalent of a theoretical plates (H), capacity factors (K), selectivity factors (α), peaks asymmetry and Resolution (Rs) were measured by analyzing different analytes on the new columns using different mobile phase compositions and flow rates. | ||
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