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Phytochemical Screening, Estimation of Some Heavy Metals Concentrations, and Specific Extraction of Bioactive Components from Iraqi Anethum graveolens L. Seeds and Studying their Antibacterial | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 5, Issue 2, 2012, Pages 28-36 PDF (214.15 K) | ||
| Authors | ||
| ; S. H. F. Al-Ma | ||
| Abstract | ||
| The current study was included the identification of bioactive components in Anethum graveolens L.. Determination of some heavy metals concentrations using Atomic Absorption Spectroscopy in Anethum graveolens L. Seeds, qualitative extraction of the most important bioactive components (Essential Oils, Glycosides, Flavonoids and Alkaloids) from Seeds, in additional to studying the antibacterial activity for these extracts against four pathogenic bacteria. The results showed a broad range of bioactive components in Anethum graveolens L. such as Tannins, Saponins, Flavonoids, Steroids, Terpenoids, Phenols, Resins, Carbohydrates, Amino Acids, Alkaloids, Glycosides and Iridoid Glycosides). The concentrations of heavy metals in Seeds recorded the values of 3.3, 10.07, 2.93, 19.05, 1.72, 1.71, 5.19, 0.72 and 4.43 (mg/ kg - dry seeds) for Chromium, Cobalt, Lead, Iron, Nickel, Copper, Zinc, Cadmium And Manganese respectively. The In vitro investigations demonstrated a disparate inhibition diameters results for the prepared Seeds extracts, the best extract with highest antibacterial activity against E.coli. was Essential Oils extract in 25 mg/ml concentration with 50 mm inhibition diameter, and the lowest one was the Alkaloids extract in 5mg/ml concentration, on other hand the highest antibacterial activity against Klebsiella Aerogenes was Essential Oils extract in 25mg/ ml concentration with 17 mm inhibition diameter, and the lowest was for Alkaloids extracts in all concentrations. | ||
| Keywords | ||
| Anethum graveolens; Heavy metals; Bioactive Components; antibacterial activity | ||
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