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Effect of Glycyrrhiza glabra extract on in vitro sperm activation and embiyonic development following intra-peritoneal insemination in mice: experimental model for mammals | ||
| Iraqi Journal of Embryos and Infertility Researches | ||
| Article 1, Volume 1, Issue 2, 2011, Pages 31-38 | ||
| Authors | ||
| saad s.aldujaily; zena muzher | ||
| Abstract | ||
| Glycyrrhizaglabm(Gg) is a herbal medicine used in the treatment of different syndromes. Recently, the effect of this plant on the reproductive system has been studied. However, its role in in vitro still not dear. objective The present study aims to investigate the possibility of using Glycyrrhiza glabra extract for in vitro sperm direct activation, and its effect on in vivo fertilization rate (FR) and early embryonic development (ED) following intra-peritoneal insemination (IPI ).The mice were used as an experimental model for mammals. Materials and methods: Glycyrrhiza glabra extract (2 mg/rnl culture medium) was used fix in vitro direct sperm activation technique. The female mice were divided into two groups: spontaneous^ ovulated (SQM) and superovulated groups (SuOM).Each group was divided into two subgroups, the first: the IPI was accomplished by epididymal sperm activated in vitro by adding 20%Gg to ihe culture medium .The second subgroup: the fP! is accomplished without adding Gg to Ihe culture medium. Revue in vitro activation of epididymal sperms with 20% Gg has shown positive effects on sperm concentration, sperm motility, and grade activity of progressive forward movement. There was a significant (P Keywords intra-peritoneal insemination --- glycyrrhize glabra --- in vitro sperm activation --- التلقيح عبر غشاء البريتون --- عرق السوس --- التنشيط في الزجاج | ||
| Keywords | ||
| intra; peritoneal insemination; glycyrrhize glabra; in vitro sperm activation; theobromine; tribulus; phoenix dactilyfera; Nasturtium officinal; contraceptive pills; pregnency; fetal testes; Achillea millefolium; Uterus; offspring | ||
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