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Genotyping and Detection of Some Virulence Genes of Klebsiella pneumonia Isolated from Clinical Cases | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 10, Issue 2, 2013, Pages 387-399 | ||
| Authors | ||
| Esraa H. Ker-Alla Al-Maamory; Jawad Kadhim Trad; Mhammad S. Abdul Razzaq | ||
| Abstract | ||
| A total of 158 samples were collected from different clinical materials (urine ,sputum, wounds and burns swabs) during the period from January to June 2012, Only 46(29.1%) isolates were identified as Klebsiella pneumonia. The molecular detecting of capsular polysaccharide gene (cps)was investigated by using PCR specific primer .It was found that only 43 isolates were positive for this marker ,whereas 3 isolates were negative to it. Genotyping of these isolates was indicated also carried out by using K1,K2 specific PCR primers. PCR technique was shown that 8 isolates were positive to K1 serotype , 14 isolates to K2 serotype, 3 isolates were positive for both K1 and K2 so they were designated as K1/K2 and 18 isolates were negative for both markers. The mag A gene was investigated, and it was found that 21 isolates were positive for mag A marker. Also ,rmpA,rmpA1 and rmpA2 markers were investigates. rmpA is found in 21 isolates, whereas rmpA1and rmpA2 were positive in 19 isolates . | ||
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