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Use latex agglutination assay technique and polymer chain reaction (PCR) in the detection of parasite Toxoplasma infection Alcondeh Toxoplasma gondii in some poultry in the city of Diwaniyah / Iraq | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 5, Issue 1, 2013, Pages 42-52 | ||
| Authors | ||
| Mohammad H; Hadi M | ||
| Abstract | ||
| The results of the laboratory tests using screening clumping latex (LAT) and the presence of infection toxoplasmosis caused by the parasite Toxoplasma Alcondeh Toxoplasma gondii in birds used in the study and proportions injury Celsius different , as shown results of the examination immune system for 150 samples of sera local chicken that 49 samples were positive , and by C infection was 32.6 % , with the highest proportion of positive 34.7 % ( 17 samples ) within the focus 801, the lowest positive rate was 2% ( one sample ) within focus 201 . As for the results of the examination of sera bathroom home Tests showed the presence of 32 sample is positive and by wounding percentage stood at 24.6% , with the highest proportion of positive 34.4 % ( 11 samples ) within the Focus 3201 and the lowest positive rate is 3.1% rate of one sample within a concentration of 201 , while in the bathroom Ataiwrani was test results and the presence of serum sample is positive and 42 injured by a percentage amounted to 32.3% . Where the highest proportion of positive 38% by 16 sample concentration within 3201 while the lowest positive rate was 4.76% by 2 concentration within the sample 201 . Been confirmed infection of poultry parasite Toxoplasma Alcondeh through the use of technology interaction chain polymers (PCR), as it has been detected gene B1 with a molecular weight (Pb325) your parasite Toxoplasma Alcondeh in 6 samples of a total of 24 samples taken at random and were incidence of 25% . The results of the current study also plays a major role in the transfer of poultry infection toxoplasmosis to humans as one of the diseases common to humans and animals and through the identification of the gene for the parasite in Msolha . The current study and identify the gene B1 (Pb325) your parasite using the PCR technique is the first in Iraq about the parasite Toxoplasma Alcondeh in pigeons . | ||
| Keywords | ||
| screening clumping latex; Toxoplasmosis; Toxoplasma gondii; PCR | ||
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