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IDENTIFICATION AND BIOCONTROL OF SOFT ROT BACTERIA DISEASE ON ONION UNDER LAB. CONDITION | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 44, Issue 3, 2013, Pages 341-347 | ||
| Authors | ||
| Abd-Aljabbar S. Ahmed; Raqib A. H. AL-Ani | ||
| Abstract | ||
| This study was conducted to identify the causal agent of onion rot disease and its control by using biological agents .The causal agent was characterized as Erwinia chrysanthemi by means of microscopic and cultural characteristics, biochemical reactions, and ability to grow on selective and differential hosts. Short rod-shaped cells with round ends, motile, and react negatively to gram-stain were observed by light microscope. Circular, convex with regular border, yellow-creamy colonies were grown on (Nutrient broth yeast extract agar) and (Yeast extract-dextrose CaCO3) media. Circular, convex, white-brownish colonies were observed on (Crystal violet-pectat medium). It has been found that E. chrysanthemi was sensitive to ethromycin, capable to reduce sucrose, liberated gas from glucose, and lysis pectin. The pathogenicity test on potato slice showed that 4 of 8 isolates obtained in this study were pathogenic. The isolate 1 was found more virulence than the other. The addition of Pseudomonas fluorescence at 108 CFUml, and agromycin at 0.5gl to the culture medium nutrient agar were led to an inhibition of E. chrysanthemi growth by 59% and 33%, respectively. | ||
| Keywords | ||
| Erwinia chrysanthemi; Onion Diseases; Biocontrol | ||
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